Qatar is urging a quick end to the war, warning that "there are no winners in the continuation of this war," Majed Al-Ansari, spokesperson for Qatar's Foreign Ministry, said at a press conference Tuesday, according to Reuters.

"We have been warning since 2023 that escalation left unchecked will get us into a situation where it cannot be controlled. And we are very close to that point. And this is why we have been urging all parties to find a resolution out of this, to find a way of ending this war before it spirals out of control," Al-Ansari said.

"A continuation of this war in this way will only mean more costs for the people of the region, for the international peace of security, for the international economy, for international energy, markets, and there are no winners in the continuation of this war. There are only losers, and the net of losers is expanding every day as a result of the ramifications of the economic situation," Al-Ansari said.

Al-Ansari also condemned all attacks on civilian infrastructure.

"Attacks on civilian infrastructure, especially energy infrastructure, is putting all of us in danger. It's affecting the wealth of the people, the prosperity of the people, and the well-being of the people, in this region and beyond," Al-Ansari said.

The Islamic Revolutionary Guard Corps warned the Gulf states on Tuesday that it will no longer show "restraint" in choosing targets for retaliation, according to the Islamic Republic News Agency.

"The U.S. regional partners should also know that until today, we have exercised great restraint for the sake of good neighborliness and have had some considerations in choosing targets for retaliation, but all these considerations have since been removed," the IRGC said.

Following President Donald Trump's threats to destroy Iran's infrastructure and intensified Israeli attacks on facilities including electricity, gas, steel, and petrochemical plants -- as well as roads and railways -- the IRGC responded, saying it would retaliate against attacks on civilian targets.

"We have not and will not be the initiators of attacks on civilian targets; but we will not hesitate to retaliate against vile attacks on civilian facilities," the IRGC said.

"The foolish leaders of America, who have thrown all their interests at the feet of the Zionists, do not even have the power to calculate what important assets of theirs are within the reach of our warriors in return for attacking our infrastructure," the statement added.

President Donald Trump issued a broad threat against Iran on Tuesday morning, as his Tuesday evening deadline loomed for Tehran to fully reopen of the Strait of Hormuz.

"A whole civilization will die tonight never to be brought back again. I don't want that to happen, but it probably will," Trump wrote on his social media platform. It was not immediately clear what the president was referring to. Trump has repeatedly threatened punitive strikes against critical Iranian infrastructure.

"However, now that we have Complete and Total Regime Change, where different, smarter, and less radicalized minds prevail, maybe something revolutionarily wonderful can happen, WHO KNOWS?" Trump also wrote.

"We will find out tonight, one of the most important moments in the long and complex history of the World. 47 years of extortion, corruption, and death, will finally end. God Bless the Great People of Iran!" the president said.

The United States struck military targets on Kharg Island overnight, according to a U.S. official.

These strikes targeted some of the same military targets struck in the earlier strike in March, though the official stressed that these strikes did not target the island’s oil infrastructure.

-ABC News' Luis Martinez